Journal: Biomedical Optics Express

Article Title: Widefield standing wave microscopy of red blood cell membrane morphology with high temporal resolution

doi: 10.1364/BOE.9.001745

Figure Lengend Snippet: Schematic diagram of experimental setup demonstrating the coupling of the 550 nm LED to the BX50 microscope system via liquid light guide and collimator. The excitation light then reflects of a >561 nm dichroic mirror to generate a SW due to self-interference at the mirror located at the specimen plane. This SW of light causes fluorescence emission at the SW anti-nodes which then propagates upwards through the lens, dichroic mirror and long pass filter to be detected by the CMOS camera. The fluorescence signals are recorded by the computer which synchronizes and triggers the LED and camera.

Article Snippet: Fluorescence emission was collected at wavelengths longer than 561 nm using a CMOS camera (ORCA-Flash 4.0LT, Hamamatsu) with a binning n = 2 and an exposure time of 33 ms.

Techniques: Microscopy, Fluorescence